Young scientists often get frustrated with laborious experimental procedures, such as cloning, PCR, or sequencing. However, the Internet has made planning and performing these procedures faster and ...

We developed an efficient "Combinatorial Strategy” for cloning different vectors with various clone sites. 1) Using originally existed clone sites from circular vectors to prepare the inserts, and if ...

Molecular cloning refers to the replication and recombination of DNA molecules. The first cloning experiments were carried out in the 1970s, when restriction endonucleases were discovered. Restriction ...

Evidence is presented that ColE1 hybrid plasmids carrying the cohesive-end site (cos) of λ can be used as gene cloning vectors in conjunction with the λ in vitro packaging system of Hohn and Murray ...

Molecular cloning is a technique used to create DNA experimental tools that are expressed in either bacterial or mammalian cells. Molecular cloning is defined as the isolation and amplification of a ...

The availability of effective vector systems is a pre-requisite for genetic manipulation of plants through recombinant DNA technology. We report here construction of a series of binary vectors that ...

As a lab within the Cooperative Center of Excellence in Hematology (CCEH) at Fred Hutch, the Vector Production core assists investigators with the design, construction, and/or production of viral ...

Whether it’s heat, emotions, or stress, the body sweats it out through a varied mix of volatile chemicals and microbes. A large language model can design and troubleshoot CRISPR protocols from scratch ...

Nereus Gunther is a molecular biologist at the Agricultural Research Service within the US Department of Agriculture (Washington D.C., USA), where part of his research focuses on Campylobacter and its ...