Restriction Enzyme Digests: Principle, Procedure, and Applications

SOLVED: You have performed a restriction digest on a linear 10.5 Kbp piece of DNA. The sizes of the DNA fragments resulting from the different restriction digests are shown below. Using this information, construct a linear map of this DNA illustrating the location(s) of the different restriction enzyme sites and the distances between each site. (20 pts)Enzyme Size of Fragments Produced (Kbp)BamHI 6.5, 2.5, 1.5EcoRI 5.5, 3.0, 2.0SalI 10.0, 0.5HindIII 8.5, 2.0BamHI/EcoRI 4.0, 2.5, 2.0, 1.5, 0.5Bam

complete the following problems restriction enzymes res which cut d na at specific sequences are classic tools in molecular biology because of their specificity in cutting dna res can be used to map d

[GET ANSWER] 7: 56 ⋯Δ(1) 4#1 Mol Bio Re...Complete the following problems.Restriction enzymes (REs), which cut D NA at specific sequences, are classic tools in molecular biology. Because of their specificity in cutting DNA, REs can be used to "map" DNA sequences by analyzing the fragments generated upon restriction digest, as in the example shown in Figure 1. Your task is to study the circular plasmid, pMBBS, through restriction digests. You subjected the pMBBS plasmid to complete digestion by

Restriction Enzyme Digests